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ABSTRACT Objective: To analyze the influence of aerobic exercise on the health of female university students. Methods: To ensure the scientificity and objectivity in the system of evaluation indexes on the quality of aerobic exercise, four first-level indexes were included: teaching purpose, teaching content, teaching method, and teaching attitude. Results: Effective improvement in body shape and function of young women could be observed. There was also improvement in the physical health of most of the women: weight reduction and body measurements were observed. Consequently, improvement in the sense of self-fulfillment, self-esteem, and encouragement of sports habits was observed. Conclusions: Factors such as eliminating excess fat and muscle strengthening contribute to the physical and psychological improvement of university students who practice aerobic physical exercises. Level of evidence II; Therapeutic studies - investigation of treatment results.
RESUMO Objetivo: Analisar a influência do exercício aeróbico na saúde das estudantes universitárias. Métodos: Para assegurar a cientificidade e objetividade no sistema de índices de avaliação sobre a qualidade do exercício aeróbico, foram incluídos quatro índices de primeiro nível: propósito de ensino, conteúdo do ensino, método de ensino e atitude de ensino. Resultados: Pode-se observar melhora efetiva na forma e função corporal das mulheres jovens. Também houve melhora na saúde física da maioria das mulheres: redução de peso e medidas corporais foram observadas. Consequentemente, foi observada a melhora na sensação de autorrealização, autoestima e estímulo aos hábitos esportivos. Conclusões: Fatores como a eliminação do excesso de gordura e fortalecimento muscular contribuem para a melhora física e psicológica das estudantes universitárias que praticam exercícios físicos aeróbicos. Nível de evidência II; Estudos terapêuticos - investigação de resultados de tratamento
RESUMEN Objetivo: Analizar la influencia del ejercicio aeróbico en la salud de las estudiantes universitarias. Métodos: Para garantizar la cientificidad y la objetividad en el sistema de índices de evaluación de la calidad del ejercicio aeróbico, se incluyeron cuatro índices de primer nivel: propósito de la enseñanza, contenido de la enseñanza, método de enseñanza y actitud de la enseñanza. Resultados: Se pudo observar una mejora efectiva en la forma y función del cuerpo de las mujeres jóvenes. También hubo una mejora en la salud física de la mayoría de las mujeres: se observó una reducción de peso y de las medidas corporales. En consecuencia, se observó una mejora en el sentimiento de realización personal, la autoestima y el estímulo de los hábitos deportivos. Conclusiones: Factores como la eliminación del exceso de grasa y el fortalecimiento muscular contribuyen a la mejora física y psicológica de los estudiantes universitarios que practican ejercicio físico aeróbico. Nivel de evidencia II; Estudios terapéuticos - investigación de resultados de tratamiento
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@#[Abstract] Objective：To explore the regulatory effect of miR-9 on biological behaviors of small cell lung cancer (SCLC) cells by targeting zinc finger E-box binding homeobox 2 (ZEB2), and to analyze the role of miR-9 in SCLC and its possible mechanism. Methods: qPCR, WB and immunohistochemistry methods were used to detect the mRNA and protein expressions of ZEB2 in cancer tissues and corresponding adjacent tissues of 67 SCLC patients who received surgical treatment at the Department of Oncology, Fourth Hospital of Hebei Medical University from February 2018 to November 2019. TargetScan was used to predict the potential target gene of miR-9, which was later verified by Dual luciferase reporter gene assay, qPCR and WB methods. CCK-8 method, Flow cytometry and Transwell experiment were used to detect the effect of miR-9 and ZEB2 over-expression on the biological behaviors of NCI-H446 cells, and WB was used to detect the protein expressions of E-cadherin, N-cadherin and Vimentin in cells. NCI-H446 cells overexpressing miR-9 were used to construct SCLC nude mouse xenograft model, and the effect of miR-9 on the growth of xenografts was observed. Results: The mRNA and protein expression levels of ZEB2 in SCLC tissues were significantly higher than those in adjacent tissues (P<0.01). There is a potential binding site on the 3' UTR of ZEB2 to bind with miR-9. Compared with the control group, the mRNA and protein expression levels of ZEB2 in NCI-H446 cells of the miR-9 over-expression group were significantly reduced (P<0.01); the proliferation, migration and invasion abilities of NCI-H446 cells were significantly suppressed (P<0.05 or P<0.01), and the expression of EMT protein was reduced; However, simultaneous over-expression of ZEB2 could reverse above effects. In in vivo experiments, the size and weight of transplanted tumors in the miR-9 over-expression group were significantly lower than those in the control group (P<0.05 or P<0.01). The expression of ZEB2 protein in the tumor tissues of nude mice in the miR-9 overexpression group was significantly lower than that in the control group (P<0.01). Conclusion: miR-9 can inhibit the biological behaviors of SCLC cells and the growth of NCI-H446 transplanted tumors in nude mice by targeting and regulating ZEB2.
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Objective@#To explore the determinants of physical activity during leisure time among junior high school students in Jinshan District of Shanghai based on socio-ecological model, so as to provide basis for health promotion in adolescents. @*Methods@#Six public junior high schools were randomly selected from Jinshan District and then a class was selected from every grade. The physical activity of the students in the spare time of one week was monitored. The determinants of physical activity were investigated through a self-designed questionnaire based on socio-ecological model,and were analyzed by optimal scaling regression. @* Results@#A total of 645 questionnaires were sent out, 622（96.43%）of which were valid. The median time spent on active physical activity after school on weekdays and at weekends was 29.00 min and 45.00 min, respectively. The scores of individual, interpersonal and environmental factors were 10.53 ± 2.04, 5.10 ± 1.51 and 8.31 ± 1.85,respectively. The results of the optimal scaling regression analysis showed that residence（β=-0.117, -0.159）,individual factors（β=0.244,0.277）, interpersonal factors（β=0.113, 0.085）and environmental factors（β=-0.140, -0.120) were the influencing factors for the physical activity in extracurricular time on weekdays and at weekends. On weekdays, the influence of individual factors was the largest （62.2%）,followed by interpersonal factors（20.8%）and residence（16.8%）. At weekends, the influence of individual factors was the largest（65.4%）, followed by residence（23.6%）and interpersonal factors（10.5%）.@*Conclusions@#The physical activity level during leisure time among junior high school students in Jinshan District is most affected by individual factors, followed by interpersonal factors and residence. The time spent on physical activity at weekends is insufficient, and the influence of residence is behind individual factors.
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Objective@#To improve physical activity by assessing pedometer-based physical activity during specific intervals over a week, among junior high school students,and to provide a reference for improving the level of students’ physical activity.@*Methods@#Students (n=675) of grades 6-8 from six public junior high schools in Shanghai were recruited and instructed to wear a pedometer for a week, step counts and contents were recorded over a specific period. This period mainly included steps taken on the way to and from school, at school, at home in the evening on weekdays, and in the morning, afternoon, and evening on weekends.@*Results@#The daily step counts recorded were 8 332 steps, with those on weekdays showing significantly higher values than step counts on weekends (9 065 steps vs. 6 392 steps)(t=22.9, P<0.01). Proportionately, the physical activity level at school contributed more to daily step counts (61.3%), followed by those on the commute to and from school(25.5%). Boys were more active than girls. For all intervals on weekdays, the step counts of students in rural districts, with overweight or obese, in grades 6 and 7, were higher than those in urban districts, with normal status, in grade 8 respectively (P<0.05). Low-activity students with physical education were more active than one without physical education(t boy=1.99,t girl=2.45,P<0.05).@*Conclusion@#These findings facilitate the implementation of effective, feasible interventions to enhance physical activity over a series of intervals during the day.
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G protein coupled receptors (GPCRs) are transmembrane receptor proteins, which allow signals to transfer across membrane. GPCRs include a large number of receptors, different receptors mediated different signaling pathways of GPCRs- adenylyl cyclase (AC)- cyclic adenosine 3' ,5'-monophosphate (cAMP), including β2 adrenergic receptors (β2- ARs)- AC- cAMP signaling pathways, E-prostanoid2/4 (EP2/4)-AC-cAMP signaling pathways. Regulatory proteins, such as G protein coupled receptor kinases (GRKs) and β-arrestins, play important modulatory roles in GPCRs signaling pathway. GPCRs signaling pathway and regulatory proteins implicate the pathogenesis process of inflammatory and immune response. Rheumatoid arthritis (RA) is an autoimmune disease characterized by synovitis and accompanied with inflammatory and abnormal immune response. This article review the advances on GPCRs signaling pathway implicating in the inflammatory and immune response of RA.
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OBJECTIVE This study was to investigate the effects of CP- 25 on the functions of activated human B cells through regulating BAFF and TNF-alpha signaling. METHODS B cells from peripheral blood mononuclear cells (PBMCs) of normal human were isolated using magnetic cell separation (MACS) by a positive selection. B cells (107 cells·mL-1) were stimulated by BAFF (100 ng·mL-1) or TNF-alpha (100 ng·mL-1) for two hours, and then were treated with CP-25 (10-5 mol·L-1) or Rituximab (5 μg·mL-1) or Etanercept (10 μg·mL-1). B cell proliferation was detected by CCK-8. B cell subsets and BAFF receptors (BAFFR, BCMA and TACI) were analyzed by flow cytometry. The expression of TNFR1 and TNFR2 on B cells was analyzed by flow cytometry. The expression of MKK3, MKK6, P-p38, P-p65, TRAF2 and p100/52 was analyzed by Western blotting. RESULTS CP-25 inhibited B cells proliferation stimulated by BAFF or TNF- alpha. CP- 25, Rituximab and Etanercept reduced the percentage and numbers of CD19+ B cells, CD19+CD20+ B cells, CD19+CD27+ B cells and CD19+CD20+CD27+ B cells induced by BAFF or TNF-alpha. CP-25 down-regulated the high expression of BAFFR, BCMA and TACI stimulated by BAFF or TNF-alpha. CP-25, Rituximab and Etanercept down-regulated significantly the expression of TNFR1 and TNFR2 on B cell stimulated by BAFF or TNF-alpha. CP-25, Rituximab and Etanercept down-regulated the expression of MKK3, P-p38, P-p65, TRAF2 and p52 in B cells stimulated by BAFF and the expression of TRAF2 and P- p65 in B cells stimulated by TNF-alpha. CONCLUSION CP- 25 regulated moderately activated B cells function by by regulating the classical and alternative NF-κB signaling pathway mediated by BAFF and TNF-alpha-TRAF2-NF-κB signaling pathway. This study suggests that CP-25 may be a promising anti-inflammatory immune and soft regulation drug.
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This study aimed to investigate the resistance mechanism of C6/36 cells to Cyt1Aa6 protein under selection pressure. Receptor binding properties of Cyt1Aa6 toward sensitive and resistant C6/36 cells were investigated. More sensitive cells were detected with goat-anti-rabbit-FITC-labeled antibody, and the quantity of in vitro activated Cyt1Aa6 toxin bound to resistant cells was greatly reduced. Ligand western blot assays showed that disappearance of the 26 kDa protein and weakness of the positive bands of 68 kDa from resistant cells might lead to the resistance of C6/36 cells to Cyt1Aa6 toxin. The resistance of C6/36 cells was detected under selection in vitro-activated Cyt1Aa6 toxin. Receptor binding demonstrated that reduced Cyt1Aa6 bound to resistant cells, which might be closely related to the disappearance and weakness of some proteins. The results presented here are the first to demonstrate that Cyt1Aa protein, a uniquely characteristic toxin, induced resistance at the cellular level. It might be attributed to the change of receptors.